P-161: Independent to Glucose and E2 the PCOS Women Show Significantly Higher Hemocysteine Level versus non PCOS

Background: Hyperhomocysteinemia is a recognized risk factor for atherosclerosis. Elevated homocysteine (Hcy) results in severe oxidative stress, platelets hyper activity, and impairs arterial circulation and smooth muscle proliferation in vascular wall. This study was carried out to evaluate follicular fluid (FF) Hcy, glucose and estradiol levels in IVF candidate PCOS women. Materials and Methods: 52 infertile patients from Dr- Tizro Day Care and IVF Center divided in to two groups including; 26 PCOS and 26 non PCOS women. Long protocol performed for all patients. FF Hcy, glucose and estradiol (E2) levels were analyzed at the time of oocyte retrieval. Automated Chemiluminescence technique (ELECYS2010HITACHI, Roche Diag. Germany, Diasorin kit), enzyme conversion immunoassay kit (Axis- Shield, Dundee, UK) and enzymatic reaction techniques (Pars Azmoon kit) were used in order to evaluate E2, Hcy and glucose respectively. Results: In PCOS patients, the FF Hcy level was manifested significantly higher in comparison to controls (p<0.001). Observations demonstrated that in PCOS group the Hcy level increased independent to E2, Glucose levels, BMI and age, while PCOS group showed significantly higher BMI in comparison to non-PCOS patients (p<0.03). The pregnancy rate in non PCOS women (50%) remarkably was higher versus PCOS group (33%). No significant differences were revealed between two groups for FF glucose and estradiol levels. Conclusion: Present data showed that although FF glucose, E2 levels were constant in PCOS and non PCOS patients, the FF Hcy levels in PCOS significantly increased.